You'll want to be comfortable so you enjoy riding more.
With that said though, you'll 'feel' more comfortable on a bike you have your heart set on.
I personally wanted to ride a cbr250rr pretty badly, and I bought one without sitting on anything else.
Also, I'm a little taller and a little lighter and the cbr seems like a nice size.
I sat on a ninja300 and sitting so upright felt awkward. I can only imagine what the wind would be like on a highway.
The new ninja250 you may find a little slow. The 300 is a bit more powerful with more torque than even my cbr, but is ~25kg heavier.
With all of this aside though, just sit on something and take a few for a test ride. If you decide on a bike before you sit on any, you'll be bias.
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